We currently provide a flow cytometry service on a BD FACS AriaIII. The technical specifications and costs are listed below. For more information, please contact Dr. Lonnie van Zyl on Tel: +27 21 959 2325, E-mail: lvanzyl@uwc.ac.za or vanzyllj@gmail.com
LASER and filter combinations available on the machine:
Near UV 375nm (2 colors): Here we offer two: DAPI/Hoechst Blue Cell cycle 450/20 NA Hoechst Red Side population 670 LP 610 LP
Violet 405nm (6 colors): a six filter combination of these: BD Horizon™ V450, Pacific Blue™, DAPI Cell surface markers 450/40 NA BD Horizon™ V500, AmCyan ” 510/50 502 LP Qdot 565 ” 575/26 545 LP Qdot 605 ” 610/20 600 LP Qdot 655 ” 660/20 630 LP Qdot 705 ” 710/50 685 LP Qdot 800 ” 780/60 735 LP
Blue 488nm (5 colors): SSC Cell surface markers, live/dead discrimination, cell cycle 488/10 NA FITC/Alexa Fluor® 488 ” 530/30 502 LP PE ” 585/42 556 LP PE-Texas Red®/PI ” 616/23 610 LP PerCP or PerCP-Cy™5.5 ” 675/20 or 695/40 655 LP PE-Cy™7 ” 780/60 735 LP
Red 633nm (3 colors): APC/Alexa Fluor® 647 Cell surface markers 660/20 NA Alexa Fluor® 700 ” 730/45 690 LP APC-Cy7 or APC-H7 ” 780/60 735 LP4.